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Centrifuges: Principles, Classifications and Applications

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  • Principle of Centrifuges
  • Classifications and Types of Centrifuges
  • Applications and Benefits of Centrifuges
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Laboratory Centrifuge

Chapter 1: Understanding the Operational Principle of Centrifuges

This chapter details the fundamental concepts of centrifuges, explaining their design, construction, and the core principles behind their operation.

What is a Centrifuge?

A centrifuge is an essential scientific tool employed to separate fluids, gases, or liquids based on varying densities. This separation occurs when the material-filled container is spun rapidly, creating centrifugal forces that propel denser substances towards the container's bottom.

Centrifuge

Centrifuge Construction

A laboratory centrifuge employs centrifugal force, generated by a rotating rotor mechanism, to segregate materials of varying particle sizes within a solution or suspension. This apparatus commonly finds use in pharmaceutical, food testing, environmental protection, and chemical analysis.

Centrifuges are crafted with an eye on design and user-friendly features, including adjustable speed and temperature controls for greater usability. A typical laboratory centrifuge comprises the following components:

Main Structure

Rotors are categorized into angular and horizontal, each designed for different speed and capacity needs. Horizontal rotors face more resistance than angular ones, making them ideal for larger volume separations rather than high-speed tasks.

Centrifuge Main Rack

Rotating Systems (Rotor)

Centrifuge rotors, whether angular or horizontal, facilitate separation at high speeds but are generally used for lower capacities. Horizontal rotors offer more resistance, more suitable for handling larger volumes than rapid separations.

Drive Mechanism

The driving system features a motor, drive shaft, and damping unit. The motor is powered by AC electricity, operating a sealed and lubricated shaft. The damping system reduces force impact, vibration, and noise, ensuring smooth functioning.

Control Interface

Managed by a microcomputer with a touch interface and digital display, the control system allows operators to adjust spin speed and centrifugal force, with options to save these settings for repeat experiments.

Centrifuge Control System

Cooling System

The refrigeration setup utilizes a sealed Copeland Compressor unit with distinct circuits for both cooling and heating control functions.

Safety Features

Security elements include protections for current, temperature, speed, balance, and door cover, ensuring safe operations.

How Centrifuges Operate

Particles in a solution separate based on density, with denser particles settling at the bottom, while lighter ones float to the top. A significant difference in densities accelerates this separation. When density differences are minimal, applying enhanced centrifugal force alongside gravity facilitates effective separation.

Centrifuge Working Principle

Operating a centrifuge involves spinning an object around a fixed axis, applying a perpendicular force. Through sedimentation, the centrifuge creates centripetal acceleration to propel denser materials to the container’s edges. Meanwhile, lighter substances collect towards the center. In laboratory conditions, samples in tubes show denser particles at the bottom with lighter ones settling above.

Operational Mechanics of Centrifuges

Centrifuges use sedimentation, combining gravitational and centrifugal forces, to sort substances by density. Techniques like isopycnic separation, pelleting, density gradient, and phase separation are employed. Among these, pelleting is most prevalent, concentrating particulate matter at the bottom as a pellet, separate from the supernatant. Chemicals are often partitioned into a solvent from an aqueous matrix during this process.

How a Centrifuge Works

Ultrafiltration employs a membrane to separate, purify, and concentrate molecules. Isopycnic centrifugation relies on sedimentation equilibrium to align particles with isodense solutions, specifying forces in gravity units rather than RPM for precision.

Key Features of a Centrifuge

Choosing the right laboratory centrifuge is an investment that requires careful consideration of the following attributes:

Peak RPM and RCF

This defines the centrifuge’s spinning prowess and force. Relative centrifugal force (RCF) is pivotal, dictating the applied force on container contents. Many centrifuges operate up to 14,000 RPM and generate an RCF of 18,624.

Centrifuge Rotor Types

Centrifuge models come with specific sets of rotors and attachments, considering:

Vessel Compatibility

The selected centrifuge should provide rotors compatible with intended vessels, available in necessary sizes.

Style of Rotor

Centrifuge models offer rotors in fixed-angle or swing-out styles, with some including additional types like vertical, PCR strip, and microlite rotors.

Maximum RCF

It’s crucial to assess if the centrifuge achieves the desired RCF.

Centrifuge Rotors

Temperature Capabilities

Centrifuges may function at room temperature or feature cooling mechanics capable of reducing temperatures to -10°C or -20°C.

Safety Characteristics of Centrifuges

Centrifuges incorporate several safety systems to safeguard users and environments. These include:

Electric Lid Lock

This function securely locks the lid during usage, avoiding unintentional exposure.

Centrifuge Electric Lid Lock

Imbalance Detection

Monitors the centrifuge’s balance, discontinuing operation if excessive vibration occurs, ensuring safety and stability.

Sealed Rotors

The design prevents biohazard spills or leaks during operation.

Rotor Identification Technology

Detects the rotor type and restricts operations within safe speed limits.

Centrifuge Safety Features

Positioning and Noise Management

When situating a centrifuge, its vibration necessitates stable placement. Additionally, being a source of noise, the unit should be placed where sound is minimized.

Centrifuge Components

All centrifuges consist of two primary components: the rotor assembly and an electric motor. The rotor assembly is responsible for creating the spin and movement transfer, while the motor converts electrical energy into mechanical and kinetic forms.

Major parts in a Centrifuge

Chapter 2: What are the Classifications and Types of Centrifuges?

This chapter explores the wide range of centrifuge classifications and types, providing insight into their designs, applications, and industry-specific uses. Understanding the differences helps buyers select the right centrifuge for their laboratory, industrial, or process-driven needs.

Classification of Centrifuges

Centrifuges are generally categorized into two main types: low-speed centrifuges and high-speed centrifuges. Each classification is distinguished by its rotational speed, capacity, technology, application scope, and specialized functionality. A clear knowledge of these categories is crucial for laboratory professionals, researchers, and production managers selecting centrifugation equipment for sample processing, material separation, or particle classification, ensuring optimal performance and reliability.

Low Speed Centrifuge

Standard low-speed centrifuges are essential in clinical laboratories, blood banks, and educational institutions for routine sedimentation and separation of heavy particles. These models typically operate at speeds ranging from 4,000 to 5,000 RPM and usually function at room temperature, with limited temperature control options. Low-speed centrifuges employ various rotor types—most commonly fixed-angle rotors and swinging bucket rotors—each designed to meet specific sample processing needs. A typical application for low-speed centrifuges is the fractionation of blood components, such as the sedimentation of red blood cells, where they are concentrated into pellets, and the supernatant is then separated by decanting. These centrifuges are also used in biochemical assays, urine analysis, and simple particle separation tasks.

Low Speed Centrifuge

High Speed Centrifuge

High-speed centrifuges are advanced laboratory and industrial machines used where precise sedimentation of minuscule particles, such as cell organelles, nucleic acids, or proteins, is required. They are employed in more advanced biochemical and clinical applications, protein purification, DNA/RNA extraction, and pharmaceutical research, where higher speeds and controlled temperatures are critical for effective sedimentation of sensitive biological samples.

High Speed Centrifuge

High-speed centrifuges can reach speeds of up to 15,000 to 20,000 RPM. Operators carefully manage both the temperature and speed to meet the specific needs of sensitive biological samples. These centrifuges typically accommodate three types of rotors: fixed-angle rotors for pelleting, swinging bucket rotors for density gradient separations, and vertical rotors for rapid and efficient separation of small particles, enhancing flexibility and throughput. Variable speed controls and advanced safety features are standard for reliable operation in demanding laboratory environments.

Types of Centrifuges

There are numerous types of centrifuges, each tailored to specific mechanical separation requirements across laboratory, industrial, pharmaceutical, and environmental sectors. The most common types include:

Basket Centrifuge

A solid wall basket centrifuge makes use of centrifugal force to promote the separation of liquids or solids. The slurry feed is brought into the rotating basket and it is made to accelerate to the basket speed. Any rather large solids or contaminants are pulled away radically from the liquid due to the centrifugal force and they collect along the inner wall of the basket.

Basket Centrifuge

The purified liquid accumulates along the interior surfaces and eventually spills over the edge, where it is then collected and allowed to exit through a gravity-fed outlet. This cycle persists until the basket is free of solids and requires emptying. Basket centrifuges are widespread in chemical processing, food production, wastewater treatment, and pharmaceutical industries due to their batch process capabilities and high solid-liquid separation efficiency.

Continuous Centrifuge

This centrifugation method allows for the processing of large volumes at high centrifugal forces without the hassle of filling numerous centrifuge tubes or frequently starting and stopping the rotors. With this approach, you'll either need to collect the supernatant or remove the pellet that has formed.

Continuous Centrifuge

Continuous centrifuges feature brief path lengths to reduce the time needed for pelleting. This design ensures efficient solid separation and allows for a swift flow of material through the rotor. Additionally, these centrifuges have large capacities, which eliminates the need for frequent starts and stops, unlike traditional rotors. This efficiency minimizes sample handling and reduces delays caused by rotor acceleration and deceleration.

Common uses for continuous flow centrifuges and continuous flow rotors include the following:

  • Large viruses
  • Mitochondria
  • Tissue homogenates
  • Bacteria
  • Cell harvest
  • Algae harvest

The continuous flow rotor is most effective when the sedimentation coefficient of the particles to be collected exceeds 50 s. With its high pelleting efficiency, this rotor separates solid material from the liquid medium more rapidly compared to swinging bucket or fixed angle rotors. It is particularly suited for samples with a solid-to-liquid ratio of 5 – 10%. For ratios above 15%, the efficiency of these rotors is even greater. Continuous centrifugation is popular in biotechnology, vaccine manufacturing, cell culture harvesting, and industrial-scale biomass processing.

Decanter Centrifuge

When it comes to separating solid or liquid mixtures with a very high solid content, the decanter centrifuge is the best choice. The finely distributed solid particles are separated out of the suspension by very strong centrifugal forces. Decanters thereby play a pivotal role in a wide variety of industries worldwide. They are optimally adapted for a specific application and the applications range from dewatering of sludge to the classification of or wet classification of solids.

Decanter Centrifuge

A decanter is a type of sedimentation vessel mounted on a rotating axis. Heavier solid particles migrate to the base of the tank due to gravity, accumulating as sediment. In contrast, a centrifuge separates solid and liquid phases using centrifugal force. The higher density solids are forced to the bottom of the centrifuge bowl, forming a sediment along the inner walls. The centrifugal force can be up to 3000 times greater than the force of gravity, compared to the standard 1g of gravitational force. Decanter centrifuges are widely implemented in wastewater treatment, oil and gas production, sludge dewatering, and the food and beverage industry for efficient continuous separation of large slurry volumes.

When choosing a decanter centrifuge, consider throughput, bowl speed, differential speed, and solids discharge mechanism to maximize operational efficiency in large-scale dewatering applications.

High Speed Centrifuge

Another type of centrifuge is the high-capacity, high-speed centrifuge. This substantial machine is floor-mounted and is typically used in laboratories that handle large volumes on a daily basis. It features a program memory capable of storing around 100 protocols and includes automatic rotor recognition. Additionally, it comes with acceleration and deceleration ramps, a spin pulse function, and lockable castors for safety. This centrifuge operates based on generating a specific centrifugal force while also considering gravitational force. The sedimentation rate is influenced by the applied centrifugal force, which acts radially outward, and depends on the following factors:

  • Angular velocity (Ꞷ measured in radians per second)
  • Radial displacement (r in cm) of the particles about the axis
  • G = Ꞷ2r

These high-speed benchtop and floor-standing centrifuges provide flexibility for multi-tasking laboratories performing routine diagnostics, genomic research, or clinical sample processing where higher gravitational forces and faster run times are required for effective separation and pelleting.

Industrial Centrifuge

Industrial centrifuges play a crucial role in dewatering and separating solids from liquids within the global mineral processing sector and various manufacturing industries. They are employed to separate solids from liquids across various industrial materials, such as sludge thickening in wastewater treatment plants, minerals recovery in mining, and purification in the chemical and pharmaceutical industries. These centrifuges are highly effective for processing a diverse array of minerals and ores, including coal, copper, and other valuable metals, helping optimize resource extraction and ensure product purity.

Industrial Centrifuge

Industrial centrifuges are generally divided into two primary categories: sedimentation centrifuges and filtration centrifuges. Sedimentation centrifuges utilize centrifugal force to separate solids from liquids, whereas filtration centrifuges apply force to push a liquid through a filtration medium. The oil and gas, pharmaceutical, food processing, and biotechnology industries often rely on these centrifuge types to support large-scale, continuous material refinement.

Examples of industrial processes that utilize centrifugation include:

  • Waste Water Processing: Separation of processed and treated water from mud and dirt, environmental wastewater sludges and some other minute substances found in the water.
  • Chemical Processing: In the production of raw products such as salts, acids, oils, and some water solids.
  • Pharmaceutical and Biotechnology: In the production of drugs, medicines, vaccines and processing of enzymatic waste.
  • Fuel and Biofuel Technology: In the synthesis of fuels like ethanol, biodiesel, lube oil, petrol refinement and biomass dewatering
  • Food Processing: It deals with the processing of vegetable oils, dairy products (such as milk, cheese), poultry, pork and beef rendering, beer, wine, and liquor clarification.
  • Mining and Mineral Processing: The process of purifying and cleansing tar sands, coal, calcium carbonate, precious metals, and kaolin clay.

When evaluating industrial centrifuge systems, it is important to consider factors such as maximum load, energy consumption, separation performance, ease of maintenance, and automation capabilities to ensure optimal production efficiency and cost savings.

Laboratory Centrifuge

This laboratory centrifuge is designed for research and medical experiments that require precise particle separation through centrifugal force. It separates particles based on factors such as density, size, shape, or viscosity by applying an artificial gravitational force. Modern lab centrifuges are equipped with digital controls, safety interlocks, multiple rotor options, and refrigeration systems to perform diverse sample preparation protocols—ranging from blood diagnostics to molecular biology techniques like DNA, RNA, and protein extraction.

Particles with higher densities or larger sizes migrate towards the bottom of the centrifuge (sedimentation), whereas those with lower densities or smaller sizes are pushed outward from the axis of rotation. This method is commonly used to separate cells, cellular components, tiny virus particles, and precipitated proteins. By providing reliable sample separation and fractionation, laboratory centrifuges are essential in clinical diagnostics, research, and pharmaceutical development.

Laboratory Centrifuge

Oil Centrifuge

The oil centrifuge, also referred to as a centrifugal oil filter, is essential for machinery maintenance, power generation, marine applications, and automotive sectors where oils need to be filtered with high efficiency. The dirty lube oil circulates from the machine into the centrifuge oil filter. The oil is then pressure driven through the filter into a bowl. The bowl is then spun at very high speeds of up to 5,000 rpm to up to 8,000 rpm with respect to the oil inlet pump pressure. The resultant gravitational force draws any particles to the exteriors of the bowl walls while the clean purified oil passes back into the machine for usage. Soot and other contaminants are repeatedly removed during regular operation of the engine.

It is important to understand that the oil filter does not use a barrier-type filtration method. Instead, it does not depend on filtration media to remove particles contaminating hydraulic or lubricant oil. This technology optimizes oil quality, reduces maintenance costs, increases equipment lifespan, and complies with ISO cleanliness standards, making it a preferred choice in heavy-duty engine maintenance and industrial lubrication systems.

Oil Centrifuge

Horizontal Peeler Centrifuge

Peeler centrifuges are batch-type units featuring perforated bowls with cloth filters. During operation, liquids are spun at high speeds, causing solids to accumulate on the filter cloth and form cakes. The cakes are then dried through a spinning process. Once dried, peeler knives scrape off the cakes, and any remaining residual material, known as heel, is removed. The scraper's position is adjusted, and high-pressure nitrogen is employed to clear the residual material. This technique is valued in the chemical and pharmaceutical fields for its gentle product handling and effectiveness in cake washing and drying processes.

A horizontal peeler centrifuge operates as a continuous flow filtration system and can be set to either automatic or manual control modes. Its automated cleaning and unloading processes ensure high levels of hygiene and product purity.

Horizontal Peeler Centrifuge

Inverting Filter Centrifuge

The inverting filter centrifuge is designed to fully expel cake material without leaving any residual heel. Like horizontal peeler centrifuges, it features a rotating drum. However, instead of using peeler knives to remove the cake, the material is pushed out through the bottom of the drum. This pushing action is so thorough that it inverts the filter cloth, effectively clearing any remaining residue and eliminating the need for mechanical cleaning.

This method ensures the highest level of product purity and is ideal for handling challenging filtration processes and situations where minimizing operator contact with the product is crucial. Its applications are prevalent in high-purity manufacturing environments, such as active pharmaceutical ingredient (API) production, specialty chemicals, and fine chemicals.

Solid Bowl Vertical Centrifuge

Solid bowl vertical centrifuges are designed for clarification by using centrifugal force to separate mixed materials. The slurry is introduced into the spinning bowl, and as the bowl’s speed increases, the centrifugal forces drive the dense, solid particles against the interior walls of the bowl, leaving the liquid phase separated. These vertical models offer compact design, low maintenance, and reliability for mineral processing, chemical refining, and pilot-scale testing.

Because solid bowl vertical centrifuges lack openings for continuous discharge, they require periodic maintenance to remove accumulated solids. This is done during the scrape cycle. Consequently, these centrifuges are not suitable for continuous flow operations as they must be halted and emptied at intervals.

Pusher Centrifuge

Pusher centrifuges, also known as horizontal basket centrifuges, are equipped with metal screening sheets or slotted sieves and utilize a hydraulic piston. These centrifuges filter liquids to form filter cakes, with the hydraulic piston providing an oscillating pushing motion to either move the cakes out of the bowl or wash them away. They are designed for continuous operation and are particularly effective for handling large volumes of solids where high purity and low residual moisture are required. Industries such as petrochemicals, minerals, plastics, and synthetics rely on pusher centrifuges for robust, reliable, and cost-effective solid-liquid separation.

In a pusher centrifuge, the basket generates centrifugal force to separate solids from the slurry. A hydraulically driven piston, which rotates with the basket, pushes the solids out with each forward stroke. Solids accumulate on the basket’s screen, while liquids pass through the metal screen or slotted sieve. This design maximizes throughput and reduces operational downtime in continuous processing lines.

Pusher Centrifuge

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    Chapter 3: What are the applications and benefits of centrifuges?

    This chapter will explore the various applications and advantages of centrifuges.

    Applications of Centrifuges

    Centrifugation encompasses a broad range of laboratory and industrial applications. This process is used to separate particles in a solution based on their viscosity, shape, size, and density. Some of the common uses of centrifuges are outlined in this section.

    Centrifugation can separate mixtures of two immiscible liquids by exploiting their density differences. It is also employed in analyzing macromolecules and their hydrodynamic properties. Additionally, it is used to fractionate various subcellular organelles and analyze membranes. Specialized centrifuges are used to purify mammalian microcells.

    In chalk production, centrifugation is used to remove water from the chalk (dewatering). Similarly, the process is employed to produce skimmed milk by separating the fat from regular milk. The centrifuge effectively separates the fat, resulting in milk with reduced fat content.

    Centrifuge Applied in Extended Spectrum

    Another practical application of centrifugation is in the stabilization and dewatering of wine. During the aging process, wine is processed in a centrifuge to enhance its stability and improve its quality. Additionally, centrifugation is utilized in forensic chemistry to analyze complex samples such as blood, DNA, urine, and other substances that are difficult to detect.

    Advantages of Centrifuges

    In recent times, centrifugation has increasingly dominated the commercial dewatering market. The following are some of the key advantages of using centrifuges.

    • Dewatering through the use of centrifuges reduces the bad odors created if the dewatering is done by traditional means. This greatly reduces olfactory nuisances and the expenses of spraying deodorants around the premises.
    • The set up can be made to function automatically if the supplied sludge is kept constant. The mechanical safety mechanisms constitute the standard equipment and to add on there are sensors that make the operations reliable.
    • A tremendous increase in the dry solids for some sludges makes it the right alternative of cleansing or purifying the substances by using centrifuges.
    • The technique is also very handy when dealing with oily sludges.
    • The centrifuges are easy to use as most devices have a microcomputer that stores settings for rotation speeds and centrifugal forces and can be saved and be used by different users.
    • The centrifugation process is relatively cheap as it is an automated process that requires minimum human attendance, thereby reducing the expenses associated with hiring manpower.
    • The centrifuge provided it is used for its intended purpose will serve a very long time as it has a rugged design, especially the decanter.

    Drawbacks Associated with Centrifuges

    Although centrifuges are highly useful, they do have their share of significant drawbacks.

    • The separation of light particles is very troublesome as the different particles are separated by size or weight. The process will be difficult for particles that have a near to zero mass as they cannot be separated easily.
    • High energy consumption as it has numerous power consuming functionalities. Temperature control consumes energy as some substances are heated first whilst some have to be cooled. Generation of high up Centrifugal forces requires the rotors to spin very fast and this has a significant kick back on the power supply.
    • Centrifuges have noise disturbances due to the high rates of revolutions per minute. Since it is a device that makes use of rotations, vibrations are inevitable and this will need noise pollution and thus rule out its usage in some locations.
    • Maintenance of the devices is very difficult and costly because a centrifuge despite its type is a very complex electronic device that requires expert servicing in cases of malfunctions.
    • In most cases a stand by unit is needed to fill in the gap when the working machine fails. Sometimes the device is used in a very crucial environment where it is expected to function. For instance, in the municipal or any water treating organizations. In the event that the device fails another centrifuge must be on standby and make sure there is an uninterrupted supply of clean water.
    • Heavy consumption of polymers

    Considerations When Choosing Centrifuges

    When considering the purchase of a centrifuge, it is important to evaluate several factors to ensure you choose the right device for your needs.

    Speed Regulation Range

    The rotor speed is an important factor to consider, as it determines the level of centrifugal force. If your work demands very high centrifugal forces, you should look for a centrifuge that offers high revolutions per minute (RPM).

    Setting Resolutions

    The centrifuge should offer programmable settings, allowing it to operate automatically without the need for manual adjustments or constant supervision.

    Rotor Imbalance

    An ideal centrifuge should be equipped with sensors to detect rotor imbalances, preventing spillage and disruptions during the separation process.

    Display Type

    The buyer should also consider the type of display required, such as Liquid Crystal Display (LCD), AMOLED, or QLED, and ensure it meets their specific needs.

    Centrifuge Display

    Overall Dimensions

    Considering the overall dimensions of the device is crucial based on its intended use. Industrial centrifuges can be as wide as 5 meters, while medical centrifuges may be as compact as 30 centimeters.

    Centrifuge Weight

    The weight of the centrifuge is another important factor. Some scientific centrifuges are compact and lightweight, whereas others can weigh up to 200 kg.

    Nominal Functioning Voltage

    The required operating voltage depends on the centrifuge’s location. Some areas use a 240V residential plug, while others require higher voltages for industrial applications. Large industrial centrifuges necessitate more voltage and are unsuitable for residential settings.

    Power Consumptions

    Centrifuges with high power consumption need a reliable and robust energy source to operate effectively without interruption.

    Work at Hand

    Before selecting a centrifuge, it’s essential to understand the specific tasks it needs to perform. Different centrifuges are designed for various purposes; some are suited for lighter tasks, such as those in pharmaceuticals, while others are built for more demanding industrial applications.

    Floor Standing Centrifuges

    Floor standing centrifuges are designed for high-speed and high-capacity operations, often reaching speeds of up to 150,000 rpm and generating forces up to 300,000N.

    Benchtop Centrifuges

    Benchtop centrifuges are compact and portable, typically suited for smaller-scale operations. Examples of these are provided below.

    Micro Centrifuges

    Micro centrifuges are intended for small volume tubes, offering relatively low speeds and forces. Some can handle both high and low temperatures, though many only provide one type of temperature control.

    Multipurpose Centrifuges

    Multipurpose centrifuges are versatile, providing a range of options and capabilities for various tasks.

    Conclusion

    A centrifuge is a scientific device that is used to separate fluids, gasses or liquids based on the density of the subject. The separation is acquired by spinning a container with the material at a very high speed. The resultant forces created will then push out the heavier materials to the extreme outside of the container.

    In summary, centrifuges are very important devices that help us in our day to day lives as they can be used in various walks of life. Ranging from laboratories, pharmaceuticals, biology and chemical technology experiments, mining, and industrial processes. There are also many different types of centrifuges with different capabilities. These centrifuges, though important, do come in with a notable number of drawbacks that need to be considered. They have a wide range of specifications that need to be considered before acquiring one.

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      Table of Contents

      Principle of Centrifuges

      Classifications and Types of Centrifuges

      Applications and Benefits of Centrifuges

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